albumin - publications

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Tris(hydroxymethyl)aminomethane-functionalized agarose particles: Parameters affecting the binding of bovine serum albumin.


J Sep Sci. 2012 Jun;35(12):1406-10


Authors: Zhang B, Wang Y, Gao M, Gu M, Wang C


Abstract

A new protein adsorbent is introduced based on the coupling of the common buffer ion, tris(hydroxymethyl)aminomethane, to the agarose gel Sepharose HPfrom GEHealthcare Bio-Sciences AB, Uppsala, Sweden. The article describes the synthesis of the new adsorbent and the use of BSA as a model in a binding study. By optimization of the coupling procedure, a maximum ligand density of 63.5 μmol/mL gel could be obtained. Adsorption equilibria were investigated in the pH range 5.0-8.0 and at salt concentrations of 0-0.4 mol/L. Binding of BSA under different conditions indicated that both electrostatic interaction and hydrogen bonding were involved in the adsorption process where the former played a major role.

PMID: 22740250 [PubMed - in process]