albumin - publications

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1. Biochem Biophys Res Commun. 2012 May 10. [Epub ahead of print]

The in vitro inhibition effect of 2 nm gold nanoparticles on non-enzymatic
glycation of Human serum albumin.

Seneviratne C, Narayanan R, Liu W, Dain JA.

Department of Chemistry, University of Rhode Island, Kingston, RI 02881, USA.

The reaction of amino groups of protein and the carbonyl groups of reducing sugar
molecules, non-enzymatically induce a series of chemical reactions that form a
heterogeneous group of compounds known as advanced glycation end products (AGEs).
The accumulation of AGEs is associated with various disease conditions that
include complications in diabetes, Alzheimer's disease and aging. The current
study monitored the extent of non-enzymatic glycation of human serum albumin
(HSA) in order to estimate the formation of HSA related AGEs in the presence of 2
nm gold nanoparticles. The rate of glycation was evaluated using several
analytical methods. Physiological concentrations of HSA and glyceraldehyde
mixtures, incubated with various concentrations of negatively charged 2 nm gold
nanoparticles, resulted in a lower reaction rate than mixtures without 2GNP.
Moreover, increasing concentrations of gold nanoparticles exhibited a pronounced
reduction in AGE formation. High performance liquid chromatography, UV-Visible
spectroscopy and circular dichroism analytical methods provide reliable
techniques for evaluating AGE formation of HSA adducts.

Copyright © 2012. Published by Elsevier Inc.

PMID: 22579685 [PubMed - as supplied by publisher]