albumin - publications

Predict more albumin - ligand interactions now!

1. Clin Exp Allergy. 2012 Feb;42(2):326-36. doi: 10.1111/j.1365-2222.2011.03934.x.

The 2S albumin allergens of Arachis hypogaea, Ara h 2 and Ara h 6, are the major
elicitors of anaphylaxis and can effectively desensitize peanut-allergic mice.

Kulis M, Chen X, Lew J, Wang Q, Patel OP, Zhuang Y, Murray KS, Duncan MW,
Porterfield HS, W Burks A, Dreskin SC.

Department of Pediatric Allergy and Immunology, Duke University Medical Center,
Durham, NC, USA.

BACKGROUND: Ara h 2 and Ara h 6, co-purified together in a 13-25 kD fraction (Ara
h 2/6; 20 kD fraction) on gel filtration chromatography, account for the majority
of effector activity in a crude peanut extract (CPE) when assayed with RBL SX-38
cells sensitized with IgE from human peanut allergic sera.
OBJECTIVES: To determine if Ara h 2/6 are the primary peanut allergens
responsible for allergic reactions in vivo and to determine if Ara h 2/6 would be
sufficient to prevent allergic reactions to a complete CPE.
METHODS: An oral sensitization mouse model of peanut allergy was used to assess
the activity of Ara h 2/6 (20 kD) and CPE without the 20 kD fraction (CPE w/o
20 kD) for allergic provocation challenge and immunotherapy. The activity of
these preparations was also tested in an assay of histamine release from human
basophils in whole blood.
RESULTS: Compared with mice challenged with control CPE, mice challenged with CPE
w/o 20 kD experienced reduced symptoms (P < 0.05) and a smaller decrease in body
temperature (P < 0.01). Results with the basophil histamine release assay
corroborated these findings (P < 0.01). The mouse model was also used to
administer Ara h 2/6 (20 kD) in an immunotherapy protocol, in which
peanut-allergic mice treated with the 20 kD fraction experienced significantly
reduced symptoms, changes in body temperature, and mast cell protease (MMCP-1)
release compared with placebo (P < 0.01 for all parameters). Importantly,
immunotherapy with the 20 kD fraction was just as effective as treatment with
CPE, whereas CPE w/o 20 kD was significantly less effective for higher dose
peanut challenges.
CONCLUSIONS AND CLINICAL RELEVANCE: Ara h 2/6 are the most potent peanut
allergens in vivo and can be used to desensitize peanut-allergic mice. These
results have potential implications for clinical research in the areas of
diagnosis and immunotherapy for peanut allergy.

© 2011 Blackwell Publishing Ltd.

PMID: 22288514 [PubMed - in process]