albumin - publications

Predict more albumin - ligand interactions now!


1. J Biomater Sci Polym Ed. 2012 Jan 24. [Epub ahead of print]

Ternary Complexes of Poly(Vinyl Pyrrolidone)-Graft-Poly(2-Dimethylaminoethyl
Methacrylate), DNA and Bovine Serum Albumin for Gene Delivery.

Zhang B, Deng L, Xing J, Yang J, Dong A.

Novel well-defined hydrophilic cationic polymers with different length of
polycation chain for gene delivery, poly(vinyl
pyrrolidone)-graft-poly(2-dimethylaminoethyl methacrylate)s (PPDs), were
synthesized by atom transfer radical polymerization (ATRP). The chemical
structures and compositions of these polymers were characterized by FT-IR,
(1)H-NMR and GPC. The experimental results of dynamic light scattering (DLS),
ΞΆ-potential and transmission electron microscopy (TEM) indicated that PPD could
condense plasmid DNA (pDNA) to form nanocomplexes. Agarose gel retardation assays
demonstrated that PPD could encapsulate plasmid DNA completely when the N/P ratio
is equal to or above 3. MTT assay and in vitro gene transfection results
indicated that PPD/pDNA complexes exhibited high transfection efficiency
concomitant with obvious cytotoxicity. Furthermore, bovine serum albumin (BSA)
was utilized to assembly with the binary complexes of PPD/pDNA to screen the
residual surface positive charges of complexes in order to decrease cytotoxicity
of the binary complexes. Physicochemical properties were characterized and the
results indicated that the coating of BSA was able to decrease the zeta potential
of the nano-sized PPD/pDNA complexes nearly to electroneutrality without
interfering with DNA condensation ability. The ternary complexes of BSA/PPD/pDNA
demonstrated no cytotoxicity and also maintained high gene transfection
efficiency in HepG2 cells in 10% serum compared with that in serum-free
condition.

PMID: 22289623 [PubMed - as supplied by publisher]