albumin - publications

Predict more albumin - ligand interactions now!


1. J Biomed Nanotechnol. 2011 Dec;7(6):743-53.

Targeted delivery of methotrexate to tumor cells using biotin functionalized
methotrexate-human serum albumin conjugated nanoparticles.

Taheri A, Dinarvand R, Atyabi F, Nouri F, Ahadi F, Ghahremani MH, Ostad SN,
Borougeni AT, Mansoori P.

Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical
Sciences, Tehran 1417614411, Iran.

Systemic toxicity following cancer chemotherapy is an important concern. Targeted
drug delivery systems could reduce the toxicity of anticancer drugs. Vitamins
have been considered as targeting moieties in novel cancer treatment strategies.
In this study, biotin was attached to nanoparticles of conjugated
methotrexate-human serum albumin. Biotin functionalized methotrexate-human serum
albumin with three different amounts of biotin attached to the nanoparticles were
prepared. It was shown that the cytotoxicity of biotin functionalized
nanoparticles on T47D and HeLa tumor cells was significantly higher than that of
free methotrexate and non-functionalized nanoparticles. The cytotoxicity of
biotin functionalized nanoparticles further increased when the number of biotin
molecules attached on the surface of nanoparticles increased. The uptake of FITC
labeled biotin functionalized nanoparticles by T47D and HeLa cells measured by
flow cytometry, was also higher than that of non-functionalized nanoparticles. It
can be concluded that the biotin functionalized methotrexate-human serum albumin
conjugated nanoparticles could be used as a potent drug for specific delivery of
methotrexate to tumors.

PMID: 22416572 [PubMed - in process]