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1. Anal Chim Acta. 2012 Mar 9;718:109-15. Epub 2012 Jan 12.

Surface imprinted thin polymer film systems with selective recognition for bovine
serum albumin.

Kryscio DR, Peppas NA.

The University of Texas at Austin, Department of Chemical Engineering, Austin, TX
78712, USA.

Molecularly imprinted polymers are synthetic antibody mimics formed by the
crosslinking of organic or inorganic polymers in the presence of an analyte which
yields recognitive polymer networks with specific binding pockets for that
biomolecule. Surface imprinted polymers were synthesized via a novel technique
for the specific recognition of bovine serum albumin (BSA). Thin films of
recognitive networks based on 2-(dimethylamino)ethyl methacrylate (DMAEMA) as the
functional monomer and varying amounts of either N,N'-methylenebisacrylamide
(MBA) or poly(ethylene glycol) (400) dimethacrylate (PEG400DMA) as the
crosslinking agent were synthesized via UV free-radical polymerization and
characterized. A clear and reproducible increase in recognition of the template
BSA was demonstrated for these systems at 1.6-2.5 times more BSA recognized by
the MIP sample relative to the control polymers. Additionally, these polymers
exhibited selective recognition of the template relative to competing proteins
with up to 2.9 times more BSA adsorbed than either glucose oxidase or bovine
hemoglobin. These synthetic antibody mimics hold significant promise as the next
generation of robust recognition elements in a wide range of bioassay and
biosensor applications.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22305905 [PubMed - in process]