albumin - publications

Predict more albumin - ligand interactions now!


1. Curr Microbiol. 2012 Mar 13. [Epub ahead of print]

Surface Display of Human Serum Albumin on Bacillus subtilis Spores for Oral
Administration.

Mao L, Jiang S, Li G, He Y, Chen L, Yao Q, Chen K.

Institute of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang,
212013, Jiangsu, People's Republic of China.

Human serum albumin (HSA) is the major protein component of human plasma. To
date, HSA for clinical uses is mostly produced by fractionation of human whole
blood, which is accompanied by a lot of limitations. To obtain long-term
bioactive albumin, we used hsa as a foreign gene and constructed a recombinant
plasmid pJS700-HSA which carries a recombinant gene cotC-hsa under the control of
cotC promoter. Plasmid pJS700-HSA was transformed into Bacillus subtilis by
double cross-over and an amylase inactivated mutant was produced. After induction
of spore formation, western blot and fluorescence immunoassay were used to
monitor HSA surface expression on spores. We estimated that HSA displayed on the
spore accounted for 0.135 % of the total spore proteins and about 0.023 fg HSA
were exposed on the surface of each spore. Oral administration to mice with
spores displaying HSA implied that the recombinant spores may have potential
ability to increase the serum albumin level in vivo due to the resistant
characters of spores.

PMID: 22411215 [PubMed - as supplied by publisher]