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Supplements in Human Islet Culture: Human Serum Albumin is Inferior to Fetal Bovine Serum.


Cell Transplant. 2012 Aug 2;


Authors: Avgoustiniatos ES, Scott WE, Suszynski TM, Schuurman HJ, Nelson RA, Rozak PR, Mueller KR, Balamurugan AN, Ansite JD, Fraga DW, Friberg AS, Wildey GM, Tanaka T, Lyons CA, Sutherland DE, Hering BJ, Papas KK


Abstract

Culture of human islets before clinical transplantation or distribution for research purposes is standard practice. At the time the Edmonton protocol was introduced, clinical islet manufacturing did not include culture and human serum albumin (HSA) was used during other steps of the process instead of fetal bovine serum (FBS) to avoid the introduction of xenogeneic material. When culture was subsequently introduced, HSA was also used for media supplementation instead of FBS that was typically used for research islet culture. The use of HSA as culture supplement was not evaluated before this implementation. We performed a retrospective analysis of 103 high-purity islet preparations (76 research preparations, all with FBS culture supplementation, and 27 clinical preparations, all with HSA supplementation) for oxygen consumption rate per DNA content (OCR/DNA, a measure of viability) and diabetes reversal rate in diabetic nude mice (a measure of potency). After 2-day culture, research preparations exhibited an average OCR/DNA 51% higher (p<0.001) and an average diabetes reversal rate 54% higher (p<0.05) than clinical preparations, despite 87% of the research islet preparations having been derived from research-grade pancreata that are considered of lower quality. In a prospective paired study on islets from 8 research preparations, OCR/DNA was on average 27% higher with FBS supplementation than with HSA supplementation (p<0.05). We conclude that the quality of clinical islet preparations can be improved when culture is performed in media supplemented with serum instead of albumin.

PMID: 22863057 [PubMed - as supplied by publisher]