albumin - publications
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1. Arch Biochem Biophys. 2012 Apr 10. [Epub ahead of print]Structural and immunological characterization of Amadori-rich human serumalbumin: Role in diabetes mellitus.Arif B, Ashraf JM, Moinuddin, Ahmad J, Arif Z, Alam K.Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh 202002, UP,India.Proteins modifications in diabetes may lead to early glycation products (EGPs) aswell as advanced glycation end products (AGEs). Whereas no extensive studies havebeen carried out to assess the role of EGPs in secondary complications ofdiabetes, numerous investigators have demonstrated the role of AGEs. Earlyglycation involves attachment of glucose on ε-NH(2) of lysine residues ofproteins leading to generation of the Amadori product (an early glycationspecies). This study reports the structural and immunological characterization ofEGPs of HSA because we believe that during persistent hyperglycemia the HSA, one of the major blood proteins, can undergo fast glycation. Glucose mediatedgeneration of EGPs of HSA was quantitated as Amadori products by NBT assay andauthenticated by boronate affinity chromatography and LC/MS. Compared to nativeHSA changes in glycated-HSA were characterized by hyperchromicity, loss influorescence intensity and a new peak in the FTIR profile. Immunogenicity ofnative- and glycated-HSA was evaluated by inducing antibodies in rabbits. Resultssuggest generation of neo-epitopes on glycated-HSA rendering it highlyimmunogenic compared to native HSA. Quantization of EGPs of HSA by authenticantibodies against HSA-EGPs can be used as marker for early detection of theinitiation/progression of secondary complications of diabetes.Copyright © 2012. Published by Elsevier Inc.PMID: 22516656 [PubMed - as supplied by publisher]