albumin - publications

Predict more albumin - ligand interactions now!


1. Phys Chem Chem Phys. 2012 Mar 1. [Epub ahead of print]

Stability and gelation behavior of bovine serum albumin pre-aggregates in the
presence of calcium chloride.

Wu H, Arosio P, Podolskaya OG, Wei D, Morbidelli M.

Institute for Chemical and Bioengineering, Department of Chemistry and Applied
Biosciences, ETH Zurich, 8093 Zurich, Switzerland. hua.wu@chem.ethz.ch.

We study, using wide-angle and small-angle light scattering techniques, the
stability and aggregation/gelation behaviors of denatured filamentous bovine
serum albumin pre-aggregates (BSA-PAs), induced by CaCl(2). It is observed that
transparent filamentous gels can be formed not only at low CaCl(2) concentrations
but also at high CaCl(2) concentrations, while turbid gels are obtained at
intermediate CaCl(2) concentrations. Although the filamentous gels at low CaCl(2)
concentrations and the turbid gels at intermediate CaCl(2) concentrations are
consistent with the literature observations, the filamentous gels at high CaCl(2)
concentrations have to be explained by different mechanisms. The latter is
attributed to the repulsive hydration interactions originating from increased
surface dipoles generated by counterion binding. Since such surface
dipole-induced hydration is very short-range and occurs mainly on charged or
polar patches of proteins (thus protected from aggregation), the aggregation of
the filamentous BSA-PAs at hydrophobic patches at the two ends is still possible,
leading to formation of the filamentous gels.

PMID: 22382723 [PubMed - as supplied by publisher]