albumin - publications

Predict more albumin - ligand interactions now!


1. Cell Microbiol. 2012 Jan 31. doi: 10.1111/j.1462-5822.2012.01757.x. [Epub ahead
of print]

SERUM ALBUMIN DISRUPTS CRYPTOCOCCUS NEOFORMANS AND BACILLUS ANTHRACIS
EXTRACELLULAR VESICLES.

Wolf JM, Rivera J, Casadevall A.

Department of Microbiology and Immunology, Albert Einstein College of Medicine,
Bronx, New York 10461.

For both pathogenic fungi and bacteria, extracellular vesicles have been shown to
contain many microbial components associated with virulence, suggesting a role in
pathogenesis. However, there are many unresolved issues regarding vesicle
synthesis and stability, including the fact that vesicular packaging for
extracellular factors involved in virulence must also have a mechanism for
vesicle unloading. Consequently, we studied the kinetics of vesicle production
and stability using [1-(14) C] palmitic acid metabolic labeling and dynamic light
scattering techniques. Cryptococcus neoformans vesicles were produced throughout
all stages of fungal culture growth and they were stable once isolated. Density
gradient analysis revealed that only a portion of the vesicle population carried
cryptococcal polysaccharide, implying heterogeneity in vesicular cargo. Vesicle
incubation with macrophages resulted in rapid vesicle instability, a phenomenon
that was ultimately associated with serum albumin. Additionally, albumin, along
with mouse serum and murine immunoglobulin destabilized Bacillus anthracis
vesicles, but the effect was not observed with ovalbumin or keyhole limpet
hemocyanin, demonstrating that this phenomenon is neither host-, microbe-, nor
protein-specific. Our findings strongly suggest that cryptococcal vesicles are
short-lived in vivo and vesicle destabilization is mediated by albumin. The
ability of albumin to promote vesicular offload through destabilization indicates
a new activity for this abundant serum protein. © 2012 Blackwell Publishing Ltd.

© 2012 Blackwell Publishing Ltd.

PMID: 22289081 [PubMed - as supplied by publisher]