albumin - publications

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Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin.


Biomed Opt Express. 2012 Sep 1;3(9):2306-16


Authors: Tellier F, Steibel J, Chabrier R, Blé FX, Tubaldo H, Rasata R, Chambron J, Duportail G, Simon H, Rodier JF, Poulet P


Abstract

Patent Blue V (PBV), a dye used clinically for sentinel lymph node detection, was mixed with human serum albumin (HSA). After binding to HSA, the fluorescence quantum yield increased from 5 × 10(-4) to 1.7 × 10(-2), which was enough to allow fluorescence detection and imaging of its distribution. A detection threshold, evaluated in scattering test objects, lower than 2.5 nmol × L(-1) was obtained, using a single-probe setup with a 5-mW incident light power. The detection sensitivity using a fluorescence imaging device was in the µmol × L(-1) range, with a noncooled CCD camera. Preclinical evaluation was performed on a rat model and permitted to observe inflamed nodes on all animals.

PMID: 23024922 [PubMed - in process]