albumin - publications

Predict more albumin - ligand interactions now!


1. Pharmazie. 2012 Feb;67(2):174-81.

Self-assembled human serum albumin-coated complexes for gene delivery with
improved transfection.

Liu N, Hao Y, Yin Z, Ma M, Wang L, Zhang X.

Laboratory of Pharmaceutics, School of Medicine, Tsinghua University, Beijing,
China.

The efficiency and safety of gene delivery vectors were important factors for
gene therapy. To enhance gene transfection efficiency and to incorporate
biocompatible components to the polyamidoamine (PAMAM) dendrimer mediated gene
delivery systems, human serum albumin (HSA) was introduced to dendriplexes of
PAMAM dendrimer and DNA via electrostatic interactions to form self-assembled
PAMAM/DNA/HSA complexes (HSA-dendriplexes). The self-assembled complexes were
characterized by gel retardation assay and particle size and zeta potential
analysis. Meanwhile, the toxicity of HSA-dendriplexes was evaluated by the MTT
assay and haemolysis test, which indicated that the complexes exhibited decreased
cytotoxicity with the incorporation of HSA. As compared to dendriplexes, the
ternary HSA-dendriplexes increased the enhanced green fluorescent protein gene
(EGFP) expression in vitro by 1.7-fold. In addition, HSA-dendriplexes showed a
significantly higher luciferase gene expression than dendriplexes or naked DNA in
the liver, kidney, lungs and spleen of mice. Our results demonstrated that
HSA-dendriplexes increases PAMAM mediated gene transfection efficiency and
decreases the cytotoxicity and haemolysis, which made the ternary complexes a
promising targeting gene delivery system.

PMID: 22512089 [PubMed - in process]