albumin - publications

Predict more albumin - ligand interactions now!

1. J Agric Food Chem. 2012 Mar 5. [Epub ahead of print]

Screening, Identification and Potential Interaction of Active Compounds from
Eucommia ulmodies Leaves Binding with Bovine Serum Albumin.

Zhang YP, Peng MJ, Liu LL, Shi SY, Peng S.

The aqueous extract of Eucommia ulmoides leaves has been commonly known as
Du-zhong tea as functional health food for treatment of hypertension,
hypercholesterolemia and fatty liver. This study developed a centrifugal
ultrafiltration-HPLC method for screening and identification of bioactive
compounds in E. ulmoides leaves binding with BSA. Six active compounds were
screened, isolated and elucidated by their UV, ESI-MS and NMR data as geniposidic
acid (1), caffeic acid (2), chlorogenic acid (3), quercetin-3-O-sambubioside (4),
rutin (5) and isoquercitrin (6). The interaction between active compounds and BSA
were investigated in the absence and presence of other compounds by quenching the
intrinsic BSA fluorescence. The results indicated the structures significantly
affected the binding process. The values of binding constants for compounds 2-6
were in the range of 105-106 mol•L-1, while geniposidic acid (1) hardly quenching
the BSA intrinsic fluorescence. However, the quenching process of geniposidic
acid was easily affected in the presence of other active compounds. The formation
of geniposidic acid-phenylpropanoid (flavonoid) complex could increase the
binding affinity of geniposidic acid with BSA, however, the increased steric
hindrance of complex may make phenylpropanoid or flavonoid dissociated from BSA,
and then decreased their affinities.

PMID: 22385161 [PubMed - as supplied by publisher]