albumin - publications

Predict more albumin - ligand interactions now!

1. Rinsho Byori. 2011 Nov;59(11):1013-8.

Redox state of urinary albumin in patients with IgA nephropathy.

Nakayama A, Odake J, Kanke A, Sakatsume M, Kasama T, Shiba K.

Faculty of Health Care Science Technology, Bunkyo Gakuin University, Bunkyo-ku,
Tokyo 113-8668.

We examined the relationship between oxidative damage and molecular instability
of urinary albumin in the urine of patients with IgA nephropathy (IgAN). As
measure of oxidation, we detected the free thiol group at Cys34 in albumin
(albumin-Cys34) using maleimide-PEG2-biotin reagent; because decreased levels of
albumin-Cys34 are correlated with increased oxidation. The urine albumin-Cys34
level in all 30 patients with IgAN was decreased to varying extents. No
correlations were found between urinary albumin/creatinine ratio and decreased
urinary albumin-Cys34 level. Furthermore, decreases in urinary albumin-Cys34 were
not accompanied by changes in serum albumin-Cys34. In diagonal-two-dimensional
SDS PAGE analysis which reveals reduction-induced degradation of H2O2-treated
human serum albumin, a similar pattern of albumin degradation was also detected
in urine samples from patients with IgAN, indicating that structural alterations
resulting from oxidative stress may be involved. Our findings suggest that redox
state, in addition to urinary albumin concentration, may be an important
indicator of post-translational modification and a potentially useful predictor
of the kidney disease.

PMID: 22352014 [PubMed - in process]