albumin - publications

Predict more albumin - ligand interactions now!


1. Biotechnol Prog. 2012 Apr 14. doi: 10.1002/btpr.1549. [Epub ahead of print]

Recombinant human albumin supports single cell cloning of CHO cells in
chemically-defined media.

Zhu J, Wooh JW, Hou JJ, Hughes BS, Gray PP, Munro TP.

The University of Queensland, Australian Institute for Bioengineering and
Nanotechnology (AIBN), Bldg.75 Cnr College and Cooper Rd., The University of
Queensland, St Lucia 4072, Australia.

Biologic drugs, such as monoclonal antibodies, are made using mammalian cells in
culture. The cell lines used for manufacturing should ideally be clonal, meaning
derived from a single cell, which represents a technically challenging process.
Fetal bovine serum is often used to support low cell density cultures, however
from a regulatory perspective it is preferable to avoid animal-derived components
to increase process consistency and reduce the risk of contamination from
adventitious agents. Chinese hamster ovary (CHO) cells are the most widely used
cell line in industry and a large number of serum-free, protein-free and fully
chemically-defined growth media are commercially available, although these media
alone do not readily support efficient single cell cloning. In this work, we have
developed a simple, fully defined, single-cell cloning media, specifically for
CHO cells, using commercially available reagents. Our results show that a 1:1
mixture of CD-CHO™ and DMEM/F12 supplemented with 1.5 g/L of recombinant albumin
(Albucult®) supports single cell cloning. This formulation can support recovery
of single cells in 43% of cultures compared to 62% in the presence of serum. ©
2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012.

Copyright © 2012 American Institute of Chemical Engineers (AIChE).

PMID: 22505017 [PubMed - as supplied by publisher]