albumin - publications

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1. J Pharm Biomed Anal. 2012 Jan 21. [Epub ahead of print]

Reactive oxygen species and nitric oxide effect on the steroid hormone binding
with serum albumin.

Aljhni R, Ibrahim F, Guillaume YC, Andre C.

Laboratoire de Chimie Analytique et Physique EA4267, Faculté de Médecine
Pharmacie, Université de Franche-Comté, Place Saint Jacques, 25030 Besançon
Cedex, France.

The interaction of a series of steroid hormones (DHEA, progesterone,
testosterone, estradiol) with serum albumin immobilized on porous silica
particles and the effect of reactive oxygen species and nitric oxide on this
interaction were studied using a biochromatographic approach. The determination
of enthalpy and entropy changes of this binding indicated that van der Waals
interactions and hydrogen bonds predominated the hormone association with
albumin. Reactive oxygen species (H(2)O(2) and OH) increased the hormone binding
affinity to albumin. On the other hand, this binding was decreased with the
presence of NO. This variation was due to conformational changes in the binding
region explained by the oxidation of some residues such as free thiol and
arginine. The thermodynamic analysis showed that free radical affects the van der
Waals forces and/or a hydrogen bond of the hormone binding with albumin. These
results explained the role of reactive oxygen species and nitric oxide in the
hormone free fraction level in the blood.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22316619 [PubMed - as supplied by publisher]