albumin - publications

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1. J Chromatogr A. 2012 Jan 18. [Epub ahead of print]

Quantification of nerve agent adducts with albumin in rat plasma using liquid
chromatography-isotope dilution tandem mass spectrometry.

Bao Y, Liu Q, Chen J, Lin Y, Wu B, Xie J.

Beijing Institute of Pharmacology and Toxicology, No. 27 Tai Ping Road, Hai Dian
District, Beijing 100850, China.

A sensitive method for the determination of the organophosphorus nerve agents
sarin, soman and VX adducts with tyrosine residue of albumin in rat plasma has
been developed and validated using liquid chromatography-isotope dilution tandem
mass spectrometry (LC-IDMS/MS). O-(O-Alkyl methylphosphonyl) tyrosine adducts and
their deuterated products that were used as the internal standards were
synthesised to establish the quantitative isotope-dilution method. Protein
purification and solid-phase extraction (SPE) were applied to improve the
recovery efficiency, reduce interference and achieve high sensitivity. The method
provided a detection limit of 0.01ng/mL for sarin and soman adducts and 0.05ng/mL
for the VX adduct. The value of the intra-day relative standard deviation over
the calibration range was less than 6.16% (n=6), and that of the inter-day was
less than 12.7% (n=6). The recovery varied from 86% to 111%. This sensitive
method was successfully applied to the analysis of adducts in rat plasma after
nerve agent exposure, and the results demonstrated the dose-effect relationships.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22305360 [PubMed - as supplied by publisher]