albumin - publications

Predict more albumin - ligand interactions now!

1. Biotechnol Prog. 2012 May 23. doi: 10.1002/btpr.1565. [Epub ahead of print]

Purification of IgG and albumin from human plasma by aqueous two phase system

Vargas M, Segura A, Herrera M, Villalta M, Angulo Y, Burnouf T, Gutiérrez JM,
León G.

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica,
San José, Costa Rica.

The current shortages in human plasma products at global levels justify the
development of new, cost effective plasma fractionation methods. We have
developed a fractionation process to obtain immunoglobulin G (IgG) and
albumin-enriched fractions based on polymer-salt aqueous two phase system (ATPS).
A small-scale (0.02 L) ATPS composed of polyethyleneglycol 3350 (PEG), potassium
phosphate and sodium chloride, at pH 6.1, was evaluated and subjected to 50-fold
scale-up (1 L). Further purification of the fractions was performed using
caprylic acid precipitation and ion exchange chromatography. Similar yield and
purity were obtained at both small and large scales. IgG precipitated in the PEG
rich upper phase at 83% recovery and 2.75-fold purification factor. An 81% pure
albumin fraction was obtained in the salt rich bottom phase with a 91% yield.
After polishing, IgG was obtained at a recovery of 70% and a purity of 92%.
Corresponding values for albumin were 91% and 90%. This IgG and albumin
fractionation technology deserves further evaluation as it may represent a
potential alternative to conventional plasma fractionation methods. © 2012
American Institute of Chemical Engineers Biotechnol. Prog., 2012.

Copyright © 2012 American Institute of Chemical Engineers (AIChE).

PMID: 22619188 [PubMed - as supplied by publisher]