albumin - publications

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1. Lasers Surg Med. 2012 Mar 13. doi: 10.1002/lsm.22015. [Epub ahead of print]

Preparation of dissolvable albumin stents for vascular anastomosis with a 1.9 µm
laser and in vitro mechanical strength assessments.

McCargar R, Jenson K, Dayton A, Murphy K, Xie H, Prahl SA.

Department of Electrical and Computer Engineering, PO Box 751 97207 Portland
State University, Portland, OR. rmccar@cecs.pdx.edu.

INTRODUCTION: We present a clinically relevant method for producing and
sterilizing dissolvable albumin stents to provide intraluminal support during
vascular anastomosis, and a method for photothermally soldering vessels using a
1.9 µm diode laser with a 200-µm spot size, albumin solder, and water as the
chromophore. Our aim in this study was to assess the mechanical integrity of
soldered vessels, and to determine if gamma-irradiation affected the solubility
of the stents. MATERIALS AND METHODS: The axial tensile strength and burst
pressure of 3.75 ± 0.3 mm inner-diameter vessels soldered with varied swath width
(1-7 mm), laser power (430-610 mW), solder concentration (22-46%w/w), and solder
layering (1-3 layers) was tested in vitro. Stent dissolution was monitored by
weight in blood, and with UV absorbance measurements in phosphate buffered saline
(PBS). Solubility was measured for stents sterilized by 25 kGy gamma-irradiation,
and stents with varied diameter and wall thickness. RESULTS: Optimized soldering
parameters yielded tensile strengths of 4.4 ± 1.2 N and burst pressures of
400 ± 90 mm Hg with stay sutures. Differences in stent solubility in blood and
PBS were not statistically significant (p = 0.99). Sterilization by 25 kGy
gamma-irradiation did not cause significant changes (p > 0.6) in stent
solubility, which was primarily volume-dependent. Under simulated intravascular
flow conditions, 3 mm stents dissolved completely with 2.7 ± 0.7 ml/mg.
CONCLUSIONS: The results show that fast-dissolving stents can be produced
reliably using the extrusion technique, and sterilized by gamma-irradiation.
Without stay sutures, soldered vessels exhibited low tensile strength, but burst
pressures comparable to sutured vessels. It was concluded that stay sutures would
be necessary in vivo due to degradation of the tensile strength of soldered
vessels with exposure to moisture. Lasers Surg. Med. © 2012 Wiley Periodicals,
Inc.

Copyright © 2012 Wiley Periodicals, Inc.

PMID: 22415571 [PubMed - as supplied by publisher]