albumin - publications

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1. Drug Test Anal. 2012 Apr 18. doi: 10.1002/dta.389. [Epub ahead of print]

Paromomycin-loaded albumin microspheres: Efficacy and stability studies.

Khan W, Kumar R, Singh S, Arora SK, Kumar N.

National Institute of Pharmaceutical Education & Research (NIPER), Nagar, India.

In the present work, paromomycin-loaded albumin microspheres (PM-MS) have been
formulated for passive targeting of paromomycin (PM) to macrophages, for the
treatment of visceral leishmaniasis (VL). PM-MS were prepared by spray-drying
method with a mean particle size of ≈ 3 µm. Thermal and chemical cross-linking
methods were used for controlling drug release from the prepared microspheres
(MS). PM-MS were then tested for efficacy and stability studies. In efficacy
study, in vitro promastigote assay was carried out to assess the susceptibility
of promastigote to PM in the concentration range of 5.0-150 µg/ml; cytotoxicity
assay was performed to determine possible toxicity of PM for the host cells
(peritoneal macrophages) and intracellular amastigote assay was carried out to
determine the efficacy of free PM (PM solution) and encapsulated PM (PM-MS).
Results obtained indicated a significant increase in efficacy of PM-MS in
comparison to PM solution at equivalent concentration. Subsequently, stability
studies of prepared formulation was carried out at various temperature and
humidity conditions, these studies provided stability of formulation at all
tested conditions including accelerated conditions. Thus, it can be concluded
that present work provides an optimized formulation with stability and enhanced
efficacy. Copyright © 2012 John Wiley & Sons, Ltd.

Copyright © 2012 John Wiley & Sons, Ltd.

PMID: 22514145 [PubMed - as supplied by publisher]