albumin - publications

1. Eur J Nucl Med Mol Imaging. 2012 Feb 17. [Epub ahead of print]

Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with
optimal retention in the sentinel lymph node.

Heuveling DA, Visser GW, de Groot M, de Boer JF, Baclayon M, Roos WH, Wuite GJ,
Leemans CR, de Bree R, van Dongen GA.

Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center,
De Boelelaan 1117, P.O. Box 7057, 1007 MB, Amsterdam, The Netherlands.

PURPOSE: At present, the only approved fluorescent tracer for clinical
near-infrared fluorescence-guided sentinel node (SN) detection is indocyanine
green (ICG), but the use of this tracer is limited due to its poor retention in
the SN resulting in the detection of higher tier nodes. We describe the
development and characterization of a next-generation fluorescent tracer,
nanocolloidal albumin-IRDye 800CW that has optimal properties for clinical SN
detection METHODS: The fluorescent dye IRDye 800CW was covalently coupled to
colloidal human serum albumin (HSA) particles present in the labelling kit
Nanocoll in a manner compliant with current Good Manufacturing Practice.
Characterization of nanocolloidal albumin-IRDye 800CW included determination of
conjugation efficiency, purity, stability and particle size. Quantum yield was
determined in serum and compared to that of ICG. For in vivo evaluation a
lymphogenic metastatic tumour model in rabbits was used. Fluorescence imaging was
performed directly after peritumoral injection of nanocolloidal albumin-IRDye
800CW or the reference ICG/HSA (i.e. ICG mixed with HSA), and was repeated after
24 h, after which fluorescent lymph nodes were excised. RESULTS: Conjugation of
IRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted
in a stable and pure product without affecting the particle size of the
nanocolloidal albumin. The quantum yield of nanocolloidal albumin-IRDye 800CW was
similar to that of ICG. In vivo evaluation revealed noninvasive detection of the
SN within 5 min of injection of either nanocolloidal albumin-IRDye 800CW or
ICG/HSA. No decrease in the fluorescence signal from SN was observed 24 h after
injection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease or
complete disappearance of the fluorescence signal was seen 24 h after injection
of ICG/HSA. Fluorescence-guided SN biopsy was very easy. CONCLUSION:
Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal
kinetic features for SN detection.

PMID: 22349719 [PubMed - as supplied by publisher]