albumin - publications
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1. Eur J Nucl Med Mol Imaging. 2012 Feb 17. [Epub ahead of print]Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer withoptimal retention in the sentinel lymph node.Heuveling DA, Visser GW, de Groot M, de Boer JF, Baclayon M, Roos WH, Wuite GJ,Leemans CR, de Bree R, van Dongen GA.Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center,De Boelelaan 1117, P.O. Box 7057, 1007 MB, Amsterdam, The Netherlands.PURPOSE: At present, the only approved fluorescent tracer for clinicalnear-infrared fluorescence-guided sentinel node (SN) detection is indocyaninegreen (ICG), but the use of this tracer is limited due to its poor retention inthe SN resulting in the detection of higher tier nodes. We describe thedevelopment and characterization of a next-generation fluorescent tracer,nanocolloidal albumin-IRDye 800CW that has optimal properties for clinical SNdetection METHODS: The fluorescent dye IRDye 800CW was covalently coupled tocolloidal human serum albumin (HSA) particles present in the labelling kitNanocoll in a manner compliant with current Good Manufacturing Practice.Characterization of nanocolloidal albumin-IRDye 800CW included determination ofconjugation efficiency, purity, stability and particle size. Quantum yield wasdetermined in serum and compared to that of ICG. For in vivo evaluation alymphogenic metastatic tumour model in rabbits was used. Fluorescence imaging wasperformed directly after peritumoral injection of nanocolloidal albumin-IRDye800CW or the reference ICG/HSA (i.e. ICG mixed with HSA), and was repeated after 24 h, after which fluorescent lymph nodes were excised. RESULTS: Conjugation ofIRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted in a stable and pure product without affecting the particle size of thenanocolloidal albumin. The quantum yield of nanocolloidal albumin-IRDye 800CW wassimilar to that of ICG. In vivo evaluation revealed noninvasive detection of the SN within 5 min of injection of either nanocolloidal albumin-IRDye 800CW orICG/HSA. No decrease in the fluorescence signal from SN was observed 24 h afterinjection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease orcomplete disappearance of the fluorescence signal was seen 24 h after injectionof ICG/HSA. Fluorescence-guided SN biopsy was very easy. CONCLUSION:Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal kinetic features for SN detection.PMID: 22349719 [PubMed - as supplied by publisher]