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MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin.

J Biomed Mater Res A. 2013 Jul 12;

Authors: Kawashita M, Hayashi J, Kudo TA, Kanetaka H, Li Z, Miyazaki T, Hashimoto M


Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2 O3 ) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2 O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2 O3 , but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2 O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2 O3 . Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.

PMID: 23853039 [PubMed - as supplied by publisher]