albumin - publications

Predict more albumin - ligand interactions now!



Liquid/Gas and Liquid/Liquid Phase Equilibria of the System Water/Bovine Serum Albumin.


J Phys Chem B. 2013 Apr 11;


Authors: Antonov YA, Eckelt J, Sugaya R, Wolf BA


Abstract

The thermodynamic behavior of the system H2O/BSA was studied at 25 °C within the entire composition range: Vapor pressure measurements via head space sampling gas chromatography demonstrate that the attainment of equilibria takes more than one week. A miscibility gap was detected via turbidity and the coexisting phases were analyzed. At 6 °C the two phase region extends from ca. 34 to 40 wt% BSA; it shrinks upon heating. The polymer rich phase is locally ordered, as can be seen under the optical microscope using crossed polarizers. The Flory-Huggins theory turns out to be inappropriate for the modeling of experimental results. A phenomenological expression is employed which uses three adjustable parameters and describes the vapor pressures quantitatively; it also forecasts the existence of a miscibility gap.

PMID: 23577649 [PubMed - as supplied by publisher]