albumin - publications

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1. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Dec 31;89C:294-300. [Epub ahead of
print]

Interaction studies of resistomycin from Streptomyces aurantiacus AAA5 with calf
thymus DNA and bovine serum albumin.

Vijayabharathi R, Sathyadevi P, Krishnamoorthy P, Senthilraja D, Brunthadevi P,
Sathyabama S, Priyadarisini VB.

Clinical Biotechnology Lab, Department of Microbial Biotechnology, Bharathiar
University, Coimbatore, India.

Resistomycin, a secondary metabolite produced by Streptomyces aurantiacus AAA5.
The binding interaction of resistomycin with calf thymus DNA (CT DNA) and bovine
serum albumin (BSA) was investigated by spectrophotometry, spectrofluorimetry,
circular dichroism (CD) and synchronous fluorescence techniques under
physiological conditions in vitro. Absorption spectral studies along with the
fluorescence competition with ethidium bromide measurements and circular
dichroism clearly suggest that the resistomycin bind with CT DNA relatively
strong via groove binding. BSA interaction results revealed that the drug was
found to quench the fluorescence intensity of the protein through a static
quenching mechanism. The number of binding sites 'n' and apparent binding
constant 'K' calculated according to the Scatchard equation exhibit a good
binding property to bovine serum albumin protein. In addition, the results
observed from synchronous fluorescence measurements clearly demonstrate the
occurrence of conformational changes of BSA upon addition of the test compound.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID: 22285949 [PubMed - as supplied by publisher]