albumin - publications

Predict more albumin - ligand interactions now!


1. Guang Pu Xue Yu Guang Pu Fen Xi. 2012 Jan;32(1):1-6.

[Interaction between three isoflavones and different isomers of human serum
albumin].

[Article in Chinese]

Ma J, Liu Y, Xie MX.

Analytical & Testing Center of Beijing Normal University, Beijing 100875, China.
maji@mail.bnu.edu.cn

The interaction mechanisms between isoflavones (Genistein, 3', 4',
7-trihydroxyisoflavone and Biochanin A) and different isomers of human serum
albumin (HSA) were investigated by fluorescence spectroscopy. Various parameters
(quenching rate constants, binding constants and number of binding sites) of
isoflavones-human serum albumin complexes were calculated. The results showed
that the isoflavones have only one binding site on human serum albumin, located
at the Site I, and the binding constants were between 0.17 x 10(5) and 1.20 x
10(5) L x mol(-1). Fluorescence enhancement experiments showed that the
fluorescence intensities of the drugs significantly increased after interacting
with HSA, indicating that the combination of drug and HSA had occurred. The
binding mechanisms between three isoflavones and HSA were discussed.

PMID: 22497114 [PubMed - in process]