albumin - publications

Predict more albumin - ligand interactions now!

1. Lipids. 2012 Jan 21. [Epub ahead of print]

Inhibition of Macrophage Oxidative Stress Prevents the Reduction of ABCA-1
Transporter Induced by Advanced Glycated Albumin.

de Souza Pinto R, Castilho G, Paim BA, Machado-Lima A, Inada NM, Nakandakare ER,
Vercesi AE, Passarelli M.

Lipids Laboratory (LIM 10), Faculty of Medical Sciences, University of São Paulo,
Av. Dr Arnaldo 455-Room 3305, São Paulo, 01246-000, Brazil.

We investigated the role of aminoguanidine and benfotiamine on the inhibition of
reactive oxygen species (ROS) generation in macrophages induced by advanced
glycated albumin (AGE-albumin) and its relationship with cell cholesterol
homeostasis, emphasizing the expression of the ATP binding cassette transporter
A-1 (ABCA-1). AGE-albumin was made by incubating fatty acid-free albumin with
10 mM glycolaldehyde. ROS production and ABCA-1 protein level were determined by
flow cytometry in J774 macrophages treated along time with control (C) or
AGE-albumin alone or in the presence of aminoguanidine or benfotiamine.
Mitochondrial function was evaluated by oxygraphy. Compared to C-albumin,
AGE-albumin increased ROS production in macrophages, which was ascribed to the
activities of NADPH oxidase and of the mitochondrial system. Mitochondrial
respiratory chain activity was reduced in cells incubated with AGE-albumin. ROS
generation along time was associated with the reduction in macrophage ABCA-1
protein level. Aminoguanidine prevented ROS elevation and restored the ABCA-1
content in macrophages; on the other hand, benfotiamine that promoted a lesser
reduction in ROS generation was not able to restore ABCA-1 levels. Inhibition of
oxidative stress induced by AGE-albumin prevents disturbances in reverse
cholesterol transport by curbing the reduction of ABCA-1 elicited by advanced
glycation in macrophages and therefore may contribute to the prevention of
atherosclerosis in diabetes mellitus.

PMID: 22271422 [PubMed - as supplied by publisher]