albumin - publications

Predict more albumin - ligand interactions now!


1. Langmuir. 2012 Apr 20. [Epub ahead of print]

In situ measurement of bovine serum albumin interaction with gold nanospheres.

Dominguez-Medina S, McDonough S, Swanglap P, Landes CF, Link S.

Here we present in situ observations of adsorption of bovine serum albumin (BSA)
on citrate-stabilized gold nanospheres. We implemented scattering correlation
spectroscopy as a tool to quantify changes in the nanoparticle Brownian motion
resulting from BSA adsorption onto the nanoparticle surface. Protein binding was
observed as an increase in the nanoparticle hydrodynamic radius. Our results
indicate the formation of a protein monolayer at similar albumin concentrations
as those found in human blood. Additionally, by monitoring the frequency and
intensity of individual scattering events caused by single gold nanoparticles
passing the observation volume, we found that BSA did not induce colloidal
aggregation, a relevant result from the toxicological viewpoint. Moreover, to
elucidate the thermodynamics of the gold nanoparticle-BSA association, we
measured an adsorption isotherm which was best described by an anti-cooperative
binding model. The number of binding sites based on this model was consistent
with a BSA monolayer in its native state. In contrast, experiments using
poly-ethylene glycol capped gold nanoparticles revealed no evidence for
adsorption of BSA.

PMID: 22515552 [PubMed - as supplied by publisher]