albumin - publications

Predict more albumin - ligand interactions now!


1. J Nanosci Nanotechnol. 2011 Dec;11(12):10765-9.

In situ comparative studies of self-assembly adsorption of bovine serum albumin
on nano films by atomic force microscopy.

Ye X, Zeng Y, Zhou T, Liu F, Liu J, Sha J, Xu X, Xia L.

College of Biomedical Engineering and Instrument Science, Zhejiang University,
Ministry of Education Key Laboratory of Biomedical Engineering, Hangzhou,
Zhejiang 310027, China.

The self-assembly adsorption of function protein on crystal surfaces, as a common
phenomenon, broadly takes place in many applications of biosensors, biocapsules
and bioMEMS/bioNEMS. To systematically investigate the different adsorption
characteristic of the same function protein on two different crystal surfaces
under the identical environment, a hybrid surface composing silica and
discontinuous Gold Nano Film (GNF) was fabricated by Physical Vapor Deposition
(PVD) and ultrasonic cleaning method, where the dynamic process of the
self-assembly adsorption of Bovine Serum Albumin (BSA) was in situ observed by
Atomic Force Microscope (AFM). The variations on the junction area of the two
different surfaces were studied in the aqueous solution before and after
injecting BSA with 0.05 mg/ml concentration. It was found that silica, compared
with same hydrophilic GNF, took on a fairly weak adsorption force. The results
indicated that the adsorption strength of BSA on the hydrophilic crystal surface
was determined not only by hydrophilic property, but also other interaction
forces, like Van der Waals and so on. Moreover, observed under the contact mode
of AFM, BSA adsorbed on GNF had great tendency to forming a ridge-like
topography. These results may be helpful in the application of immunosensors and
other areas.

PMID: 22408991 [PubMed - in process]