albumin - publications

Predict more albumin - ligand interactions now!


1. Drug Dev Ind Pharm. 2012 May 17. [Epub ahead of print]

Implementation of mixture design for formulation of albumin containing
enteric-coated spray-dried microparticles.

Shastri PN, Ubale RV, D'Souza MJ.

Mercer University, Nanotechnology Laboratory, College of Pharmacy and Health
Sciences , Atlanta, GA 30341 , USA.

Context: Oral delivery of proteins has been a challenging as well as rapidly
developing field. Objective: To implement mixture design of experiment to develop
enteric-coated microparticles containing bovine serum albumin. Materials and
methods: Microparticles were prepared using Buchi Spray Dryer 191. Simplex
lattice mixture design computed using JMP software was implemented to compare the
gastric protection rendered by Eudragit FS30D, Eudragit L100-55, and Eudragit
S100 in microparticulate form. Further, an extreme vertices mixture design was
used to incorporate hydroxypropyl methylcellulose (HPMC) Chitosan in the
formulation to delay the release. Microparticle recovery yield and protein
content in microparticles were evaluated. Results and discussions: The design was
statistically significant with Eudragit S100 resulting in protein release of < 5%
in acidic buffer. The selected optimal formulation had 70% of Eudragit S, 25%
HPMC, and 5% Chitosan. The release profiles of protein from Eudragit S alone and
along with HPMC were compared. About 25% decrease in the amount of protein
release was observed 6 h post exposure of microparticle to buffer of pH 6.8. The
microparticle recovery yield reduced from 77.99% to 71.56% which is due to
addition of HPMC into the formulation matrix. Conclusion: Although all three
Eudragit polymers can be used for enteric coating, in the microparticulate form
Eudragit S resulted in higher gastric protection. Also use of HPMC along with
Eudragit S resulted in further sustained release.

PMID: 22591196 [PubMed - as supplied by publisher]