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Immobilization of bovine serum albumin-protected gold nanoclusters by using polyelectrolytes of opposite charges for the development of the reusable fluorescent Cu(2+)-sensor.


Biosens Bioelectron. 2013 Jan 16;44C:16-20


Authors: Su L, Shu T, Wang Z, Cheng J, Xue F, Li C, Zhang X


Abstract

In this paper, we reported a facile method of fabricating a reusable fluorescent Cu(2+)-sensor. To fabricate the reusable sensor, the bovine serum albumin-protected gold nanoclusters (BSAGNCs) were complexed with polyelectrolytes, i.e., positively charged polydiallyldimethylammonium (PDDA) and negatively charged polystyrenesulfonate (PSS), and were coated on a glass slide. The prepared film-modified glass slides exhibited the recyclability of fluorescent signal "off-on" behaviors: the fluorescence could be switched "off" by immersing the film sensors in Cu(2+) solution and "on" by washing with ethylene diamine tetraacetic acid (EDTA) solution. The prepared film-modified glass slides exhibited high selectivity towards Cu(2+) the fluorescence quenching behavior in the form of the Stern-Volmer equation. In addition, the spiked tap water samples were analyzed with satisfactory results. These demonstrations provide a new convenient approach to develop the BSAGNCs-based, reusable fluorescence sensors.

PMID: 23384766 [PubMed - as supplied by publisher]