albumin - publications

Predict more albumin - ligand interactions now!

1. Biomed Chromatogr. 2012 Feb 15. doi: 10.1002/bmc.2708. [Epub ahead of print]

Identification of modification sites on human serum albumin and human hemoglobin
adducts with houttuynin using liquid chromatography coupled with mass

Deng Z, Zhong D, Chen X.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai,
201203, China.

Houttuynin, a β-keto aldehyde compound, is the major active ingredient in herba
houttuyniae injection. The injection was once used as an anti-inflammatory drug
associated with occasional serious hypersensitivity reactions in the clinic,
which were proposed to be related to the formation of protein adducts. N(α)
-Boc-lysine, FEEM and IVTNTT were used as model amino acids or peptides
containing one nucleophilic residue to investigate adduct types by liquid
chromatography coupled with ion trap mass spectrometry (LC/MS(n) ) and
high-resolution quadrupole time-of-flight mass spectrometry (Q-TOF MS). These
adducts were respectively characterized as Schiff bases formed by 1:1 reaction of
houttuynin with lysine or N-terminal residue and pyridinium adducts by 2:1
reaction. LC/MS(n) analysis of trypsin digests of HSA/Hb incubations with
houttuynin revealed that houttuynin-modified HSA adducts were formed mainly at
N-terminal amino acid and lysine residues, specifically at Lys-212, Lys-414 and
Lys-525 for Schiff base adducts, and at Lys-414 and Lys-432 for pyridinium
adducts, and houttuynin adducted more readily with N-terminal valine of the α-
and β-chains in Hb and lysine amine (Lys-62) of the β-chain for Schiff base
adducts. The results showed the direct modification of houttuynin to HSA/Hb in
vitro, which was speculated to be responsible for the adverse reactions induced
by houttuyniae injection. Copyright © 2012 John Wiley & Sons, Ltd.

Copyright © 2012 John Wiley & Sons, Ltd.

PMID: 22334394 [PubMed - as supplied by publisher]