albumin - publications

Predict more albumin - ligand interactions now!


1. Appl Biochem Biotechnol. 2012 Feb 11. [Epub ahead of print]

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA)
Based on a Pair of Specific Monoclonal Antibodies and
Peroxyoxalate-glyoxaline-PHPPA Dimer Chemiluminescent System in Vaccines.

Xue P, Zhang K, Zhang Z, Li Y, Liu F, Sun Y, Zhang X, Song C, Fu A, Jin B, Yang
K.

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,
School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an,
710062, China.

Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed
fluorescent reaction, and oxalate chemiluminescence analysis have been combined
to develop a highly sensitive, simple, and rapid method for analysis of bovine
serum albumin (BSA) based on a pair of specific monoclonal antibodies in
vaccines. A typical "sandwich type" immunoassay was used. Reaction of
3-(4-hydroxyphenyl propionate) (PHPPA) with hydrogen peroxide-urea, catalyzed by
HRP, produced fluorescence of 3-(4-hydroxyphenyl propionate) dimer, which was
detected by chemiluminescence analysis with the bis(2,4,6-trichlorophenyl)oxalate
(TCPO)-H(2)O(2)-glyoxaline-PHPPA dimer chemiluminescent system. This method
exhibited high performance with a linear correlation between response and amount
of bovine serum albumin (BSA) in the range 0.1 to 100.0 ng mL(-1) (r = 0.9988),
and the detection limit was 0.03 ng mL(-1) (S/N = 3). Intra- and interassay
coefficient variations were all lower than 9.0% at three concentrations (1.0,
20.0, and 80.0 ng mL(-1)). The proposed method has been used for successful
analysis of the amount of residual BSA in vaccines. The results obtained compared
well with those obtained by conventional colorimetric ELISA and luminol
chemiluminescent ELISA.

PMID: 22328250 [PubMed - as supplied by publisher]