albumin - publications

Predict more albumin - ligand interactions now!

1. Anal Chim Acta. 2012 Mar 16;719:61-7. Epub 2012 Jan 8.

Fast screening of terpenes in fragrance-free cosmetics by fluorescence quenching
on a fluorescein-bovine serum albumin probe confined in a drop.

Cabaleiro N, de la Calle I, Bendicho C, Lavilla I.

Departamento de Química Analítica y Alimentaria, Área de Química Analítica,
Facultad de Química, Universidad de Vigo, Campus As Lagoas-Marcosende s/n, 36310
Vigo, Spain.

A headspace single drop microextraction procedure is proposed for terpene
screening in fragrance-free cosmetics. The drop is composed by an aqueous
solution of a fluorescence probe formed by bovine serum albumin and fluorescein.
Extracted volatile terpenes produce a fluorescence quenching that can be
monitored by microvolume-fluorospectrometry. This quenching is observed on the
fluorescein fluorescence only when it is linked to bovine serum albumin. A
mechanism of contact quenching is proposed. Variables related to the terpene
microextraction procedure were carefully studied, namely drop composition and
volume, microextraction time, sample volume and temperature, stirring rate and
salt addition. The only sample treatment is the dilution of cosmetic with 40%
(v/v) ethanol. Citronellol was selected as a representative terpene for
calibration purposes. According to the European legislation, the
probability-concentration graph of the screening system was established using
0.001% (w/w) as the cut-off level. Low limits of detection with simple
instrumentation, absence of matrix effects and high sample throughput can be

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22340532 [PubMed - in process]