albumin - publications

Predict more albumin - ligand interactions now!


1. Zhonghua Gan Zang Bing Za Zhi. 2012 Jan;20(1):50-4. doi:
10.3760/cma.j.issn.1007-3418.2012.01.014.

[Establishment of an albumin and cytokeratin 19 genetically-modified embryonic
stem cell line and evaluation of its hepatoblast differentiation capacities].

[Article in Chinese]

Lan Y, Li YF, Li DJ, Wei JM, Wang X, Hu HP.

Department of General Surgery, Beijing Hospital, Beijing 100730, China.

OBJECTIVE: To establish a gene-modified embryonic stem (ES; E14.1-2) cell line
with hepatoblast differentiation reporter genes, albumin (ALB) and cytokeratin 19
(CK19), labeled to facilitate study of their potential applicability as
differentiated hepatoblasts.
METHODS: Two expression vectors were constructed, one with the ALB promotor
driving the enhanced green fluorescent protein (EGFP) and anti-neomycin genes
(pAlb-EGFP), and the other with the CK19 promotor driving the red fluorescence
protein and anti-hygromycin genes (pCK19-hCD25-IRES-tdTOMATO). The linearized
vectors were electroporated into the E14.1 line, and double reporter
genes-modified ES cells (E14.1-2) were selected by neomycin and hygromycin.
E14.1-2 hepatoblast differentiation was indcued by exposure to growth factors
(BMP4 and bFGF) and evidenced by embryoid body formation. Fluorescence-activated
cell sorting (FACS) and reverse transcription-polymerase chain reaction (RT-PCR)
were used to confirm whether differentiated cells were hepatoblast-like and to
quantify the differentiation efficiency. RSULTS: The pAlb-EGFP and
pCK19-hCD25-IRES-tdTOMATO vectors were shown to specifically activate ALB and
CK19 exprssion. The E14.1-2 cell line with labeled ALB and CK19 was established,
and shown to have pluripotency by RT-PCR detection of pluripotent markers'
expression, namely Oct4 and SSEA-1. After 22 days of induction, 21.27% of the
differentiated hepatoblasts were detected by FACS as positive for ALB and CK19
expression.
CONCLUSIONS: A gene-modified ES cell line was generated with hepatocyte
differentiation reporter genes ALB and CK19 labeled. The differentiation of the
resultant E14.1-2 line was technically simple to qualify and quantify, and will
likely aid future studies of hepatoblast characteristics.

PMID: 22464707 [PubMed - in process]