albumin - publications

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1. Curr Drug Deliv. 2011 Nov 1;8(6):678-90.

Effect of Sugar Additives on Stability of Human Serum Albumin during Vacuum Foam
Drying and Storage.

Hajare AA, More HN, Pisal SS.

Department of Pharmaceutical Technology, Bharati Vidyapeeth College of Pharmacy,
Kolhapur (MS), India. aahajare@rediffmail.com.

No literature on the protein stabilization of human serum albumin (HSA) by vacuum
foam drying (VFD) has been reported. The purpose of this study was to investigate
the effect of sugar-additive systems on the stability of HSA by VFD. For the
assessment, HSA was formulated with sucrose and mannitol, respectively, alone or
in combination with stabilizers, which were vacuum foam dried and stored at 25C.
Protein content of the resulting dried formulations was analyzed by Lowry method.
Fourier-transform infrared spectroscopy (FT-IR) analysis of the HSA secondary
structure showed apparent protein structure-stabilizing effects of the amorphous
sugar and phosphate combination during the VFD. In particular, sucrose-sodium
phosphate monobasic mixture provide an interesting alternative to pure saccharide
formulations due to their high glass transition temperatures and their increased
ability to maintain a low melting transition temperature in the presence of small
amounts of water. Inhibition of the sucrose crystallization in solutions under
vacuum resulted in highly amorphous sucrose. Changes in the endothermic melting
transition suggested reduced sucrose molecular mobility with increase in the
sodium phosphate ratio. The addition of phosphate salts to sugar systems has
several interesting features that merit its consideration in formulations to
protect dehydrated labile biomaterials. In conclusion, our data suggest that
sucrose and phosphate as additives seem to protect HSA during VFD better than
lyophilized products and also maintain its stability in the VFD state during
storage.

PMID: 22313163 [PubMed - in process]