albumin - publications

Predict more albumin - ligand interactions now!


1. Int J Biol Macromol. 2012 Apr 26. [Epub ahead of print]

Effect of polyethylene glycols on the alkaline-induced molten globule
intermediate of bovine serum albumin.

Qu P, Wang Y, Wu G, Lu Z, Xu M.

Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry,
College of Chemistry and Chemical Engineering, Shangqiu Normal University, Wenhua
Road 298#, Shangqiu 476000, PR China; State Key Laboratory of Bioelectronics,
School of Biological Science & Medical Engineering, Southeast University, Si Pai
Lou 2#, Nanjing 210096, PR China.

In the present study, the formation of one molten globule-like unfolding
intermediate of bovine serum albumin (BSA) at pH 11.2 has been established with
the help of circular dichroism (CD) spectra, fluorescence spectroscopy and 'phase
diagram' approach. Additionally, we have shown the conformational changes
occurring in the pH 11.2 intermediate of BSA when it was exposed to different
molecular weight polyethylene glycols (PEGs) at varying concentrations. When the
pH 11.2 intermediate of BSA was treated by PEG 400 there was induction of
secondary and non-native tertiary contacts. In case of PEG 8000 and PEG 20,000,
the loss in secondary as well as tertiary structure was observed. PEG 8000 and
20,000 altered the conformation of the pH 11.2 intermediate and resulted in its
transition to another intermediate state in which the hydrophobic patches were
inaccessible.

Copyright © 2012. Published by Elsevier B.V.

PMID: 22561740 [PubMed - as supplied by publisher]