albumin - publications

Predict more albumin - ligand interactions now!


1. J Sep Sci. 2012 May 3. doi: 10.1002/jssc.201101020. [Epub ahead of print]

Dye attached poly(hydroxyethyl methacrylate) cryogel for albumin depletion from
human serum.

Andac M, Galaev I, Denizli A.

Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara,
Turkey.

Cibacron Blue F3GA was immobilized on poly(hydroxyethyl methacrylate) cryogel and
it was used for selective and efficient depletion of albumin from human serum.
The poly(hydroxyethyl methacrylate) was selected as the basic component because
of its inertness, mechanical strength, chemical and biological stability, and
biocompatibility. Cibacron Blue F3GA was covalently attached to the
poly(hydroxyethyl methacrylate) cryogel to produce poly(hydroxyethyl
methacrylate)-Cibacron Blue F3GA cryogel affinity column. The poly(hydroxyethyl
methacrylate)-Cibacron Blue F3GA cryogel was characterized with respect to
gelation yield, swelling degree, total volume of macropores, Fourier Transform
Infrared spectroscopy, and scanning electron microscopy. It was found that the
maximum amount of adsorption (343 mg/g of dry cryogel) obtained from experimental
results is very close to the calculated Langmuir adsorption capacity (345 mg/g of
dry cryogel). The maximum adsorption capacity for poly(hydroxyethyl
methacrylate)-Cibacron Blue F3GA cryogel column was obtained as 950 mg/g of dry
cryogel for nondiluted serum. The adsorption capacity decreased with increasing
dilution ratios while the depletion ratio of albumin remained as 77% in serum
sample. Finally, the poly(hydroxyethyl methacrylate)-Cibacron Blue F3GA cryogel
was optimized for using in the fast protein liquid chromatography system for
rapid removal of the high abundant proteins from the human serum.

© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID: 22556120 [PubMed - as supplied by publisher]