albumin - publications

Predict more albumin - ligand interactions now!

1. Colloids Surf B Biointerfaces. 2012 Jan 13. [Epub ahead of print]

Diffusion of rhodamine B and bovine serum albumin in fibrin gels seeded with
primary endothelial cells.

Shkilnyy A, Proulx P, Sharp J, Lepage M, Vermette P.

Laboratoire de bio-ingénierie et de biophysique de l'Université de Sherbrooke,
Department of Chemical and Biotechnological Engineering, Université de
Sherbrooke, 2500, boul. de l'Université, Sherbrooke, QC, Canada J1K 2R1.

Scaffolds with adequate mass transport properties are needed in many tissue
engineering applications. Fibrin is considered a good biological material to
fabricate such scaffolds. However, very little is known about mass transport in
fibrin. Therefore, a method based on the analysis of fluorescence intensity for
measuring the apparent diffusion coefficient of rhodamine B and
fluorescein-labelled bovine serum albumin (FITC-BSA) is described. The
experiments are performed in fibrin gels with and without human umbilical vein
endothelial cells (HUVEC). The apparent diffusion coefficients of rhodamine B and
FITC-BSA in fibrin (fibrinogen concentration of 4mg/mL) with different cell
densities are reported. A LIVE/DEAD(®) assay is performed to confirm the
viability of HUVEC seeded at high densities. Diffusion coefficients for rhodamine
B remain more or less constant up to 5×10(5)cells/mL and correlate well with
literature values measured by other methods in water systems. This indicates that
the presence of HUVEC in the fibrin gels (up to 5×10(5)cells/mL) has almost no
effect on the diffusion coefficients. Higher cell densities (>5×10(5)cells/mL)
result in a decrease of the diffusion coefficients. Diffusion coefficients of
rhodamine B and FITC-BSA obtained by this method agree with diffusion
coefficients in water predicted by the Stokes-Einstein equation. The experimental
design used in this study can be applied to measure diffusion coefficients in
different types of gels seeded or not with living cells.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22293601 [PubMed - as supplied by publisher]