albumin - publications

Predict more albumin - ligand interactions now!

1. Ann Clin Biochem. 2012 Apr 5. [Epub ahead of print]

Development of an automatic laboratory computer flagging system to identify urine
albumin samples potentially affected by antigen excess ('hooking').

Pullan NJ, Hitch T.

Clinical Pathology, Nottingham University Hospitals NHS Trust (QMC Campus),
Nottingham NG7 2UH, UK.

BACKGROUND: Immunoassays for urinary albumin are often subject to the problem of
antigen excess (the 'hook' effect) at high albumin concentrations. We developed
an automated protocol to identify such samples based on urinary albumin to
creatinine ratio (uACR) and urinary total protein (uTP) results. METHODS: An
automated flagging system was designed and written into the laboratory computer
system to alert technical staff to samples potentially affected by the 'hook
effect'. This flag was activated when there was a combination of an uTP of ≥2400
mg/L and an uACR of <30 mg/mmol. RESULTS: The potential rate of false-negative
uACR results was approximately 0.17% in samples from primary care and diabetic
clinic sources. CONCLUSIONS: Samples with falsely low uACR results were
identified, allowing the vast majority of results to be authorized without
intervention. The protocol prevented the reporting of false-negative uACR results
which might impact on the management of patients.

PMID: 22492878 [PubMed - as supplied by publisher]