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Comparison of 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) Levels Using Mass Spectrometer and Urine Albumin Creatinine Ratio as a Predictor of Development of Diabetic Nephropathy.


Free Radic Res. 2012 Jul 13;


Authors: Serdar M, Sertoglu E, Uyanik M, Tapan S, Akin K, Bilgi C, Kurt I


Abstract

Abstract Background: Measurement of urinary 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) has recently become more popular as a means of assessing oxidative stress in the human body. The aim of this study is to compare the levels of urine 8-OHdG in type 2 diabetes with and without nephropathy and to evaluate its role as a biochemical marker for distinguishing these patients from healthy and patients without complications. Methods: For this purpose, 52 patients with type 2 diabetes mellitus (32 with nephropathy (DMN), 20 without nephropathy (DM)) and 20 healthy control subjects (C) were included in this study. The urine concentrations of 8-OHdG were measured by modified LC-MS/MS method and compared with the first morning voiding urine albumin creatinine ratio and HbA1c values of the same patients. Results: The concentrations of urine 8-OHdG in DMN and DM were higher than the control subjects (3.47±0.94, 2.92±1.73, 2.1±0.93 nmol/mol creatinine, respectively). But there was no statistically difference between DMN and DM (p=0.115). There is significant correlation between urinary 8-OHdG and urine albumin creatinine ratio (r=0.501, p<0.001). According to ROC analysis, the AUC value of HbA1c was higher than the value of the AUC of 8-OHdG (respectively, 0.882, 0.771) Conclusions: This study shows that the urine 8-OHdG levels increase in diabetic patients. However, urinary 8-OHdG is not a useful clinical marker compared to urine albumin creatinine ratio to predict the development of diabetic nephropathy in diabetic patients.

PMID: 22789030 [PubMed - as supplied by publisher]