albumin - publications

Predict more albumin - ligand interactions now!


1. Talanta. 2012 May 15;93:239-44. Epub 2012 Feb 17.

Characterization of interactions between organotin compounds and human serum
albumin by capillary electrophoresis coupled with inductively coupled plasma mass
spectrometry.

Sun J, He B, Liu Q, Ruan T, Jiang G.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research
Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box
2871, Beijing 100085, China.

Thermodynamic data such as the binding constants are vital parameters describing
interactions between exotic trace compounds and biomolecules in biochemical
property modeling. In this study, the stability constants of organometallic
compound and protein complexes were studied by using capillary electrophoresis
coupled with inductively coupled plasma mass spectrometry (CE-ICP-MS),
considering its low detection limits and low sample demand. Four organotin
compounds (trimethyltin (TMT), tripropyltin (TPrT), tributyltin (TBT),
triphenyltin (TPhT)) and human serum albumin (HSA) were used as model
organometallic compounds and protein, respectively. Affinity capillary
electrophoresis (ACE) and nonequilibrium capillary electrophoresis assays of
equilibrium mixtures (NECEEM) were performed and compared by using ICP-MS as the
detector to determine the binding constants of organotin compounds and HSA in 1:1
molar ratio assumption. Constant measurements of the two methods were both
simple, however, ACE assays were more accurate and more appropriate for the
constant determination of the organotin-HSA complexes, considering the errors of
the NECEEM method. A good precision of the binding constants (logK(b)) using the
ACE method was proved by different mathematical calculations, and the values were
6.13±0.51 (TMT), 5.72±0.38 (TPrT), 5.68±0.34 (TBT), 6.05±0.38 (TPhT) respectively
for each of the organotin-HSA complexes, showing non-covalent interaction between
organotin compounds and HSA. Meanwhile, this study also confirms the suitability
of CE-ICP-MS method for further studies on organometallic complexation.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22483905 [PubMed - in process]