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Characterization and analytical application of Morin - Bovine serum albumin system by spectroscopic approaches.


Spectrochim Acta A Mol Biomol Spectrosc. 2012 Sep 10;


Authors: Wang F, Huang W, Miao X, Tang B


Abstract

It is found that the fluorescence intensity of Morin can be strongly quenched by proteins. Based on this, a new fluorimetric method for the determination of protein was developed. Under optimum conditions, the quenchment of Morin fluorescence was in proportion to the concentration of proteins in the range 0.0001-0.1000g·L(-1) for bovine serum albumin (BSA) and 0.0005-0.1000g·L(-1) for human serum albumin (HSA). The reaction mechanism indicates that proteins can bind with Morin at the 3-hydroxyl and the 4-carbonyl and form a non-fluorescence complex 4:1molar ratio of Morin/BSA, which results in the fluorescence of Morin and BSA are all quenched.

PMID: 23031639 [PubMed - as supplied by publisher]