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Catalytic formation of silver nanoparticles by bovine serum albumin protected-silver nanoclusters and its application for colorimetric detection of ascorbic acid.

Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jan 17;106C:224-230

Authors: Yang XH, Ling J, Peng J, Cao QE, Wang L, Ding ZT, Xiong J


We established a simple spectrophotometric and colorimetric method for detection of ascorbic acid based on the growth of silver nanoparticles in bovine serum albumin protected-silver nanoclusters (BSA-AgNCs) and Ag(+) mixture. Due to the catalysis of BSA-AgNCs, ascorbic acid could reduce Ag(+) to silver nanoparticles (NPs) at room temperature. The color of the mixture changed from colorless to yellow and a strong absorption band near 420nm could be found in their absorption spectra owing to localized surface plasmon resonance (LSPR) of produced silver NPs. The absorbance changes at 420nm had a good relationship with ascorbic acid concentration. Thus, we proposed a spectrophotometric and colorimetric method to determine ascorbic acid in concentration range from 2.0 to 50.0μM, with the corresponding limits of determination (3σ) of 0.16μM.

PMID: 23396009 [PubMed - as supplied by publisher]