albumin - publications

Predict more albumin - ligand interactions now!

1. Colloids Surf B Biointerfaces. 2012 Feb 1. [Epub ahead of print]

Bovine serum albumin film as a template for controlled nanopancake and nanobubble
formation: In situ atomic force microscopy and nanolithography study.

Kolivoška V, Gál M, Hromadová M, Lachmanová S, Tarábková H, Janda P, Pospíšil L,
Turoňová AM.

J. Heyrovský Institute of Physical Chemistry of ASCR, v.v.i., Dolejškova 3, 182
23 Prague, Czech Republic.

Air nanobubbles and nanopancakes were investigated in situ by both tapping mode
atomic force microscopy (TM AFM) and atomic force nanolithography techniques
employing bovine serum albumin (BSA) film supported by highly oriented pyrolytic
graphite (HOPG). The BSA denaturation induced by the water-to-ethanol exchange
served for conservation of nanobubble and nanopancake sites appearing as imprints
in BSA film left by gaseous cavities formerly present on the interface in the
aqueous environment. Once the BSA film was gently removed by the nanoshaving
technique applied in ethanol, a clean basal plane HOPG area with well-defined
dimensions was regenerated. The subsequent reverse ethanol-to-water exchange led
to the re-formation of nanopancakes specifically at the nanoshaved area. Our
approach paves the way for the study of gaseous nanostructures with defined
dimensions, formed at solid-liquid interface under controlled conditions.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22341519 [PubMed - as supplied by publisher]