albumin - publications

Predict more albumin - ligand interactions now!

1. Environ Sci Technol. 2012 Jan 23. [Epub ahead of print]

Bovine Serum Albumin adsorption to Iron-oxide coated sands can change microsphere
deposition mechanisms.

Flynn RM, Yang X, Hofmann T, Von Der Kammer F.

Particulate colloids often occur together with proteins in sewage-impacted water.
Using Bovine Serum Albumin (BSA) as a surrogate for protein in sewage, column
experiments investigating the capacity of iron-oxide coated sands to remove latex
microspheres from water revealed that microsphere attenuation mechanisms depended
on antecedent BSA coverage. Dual pulse experiment (DPE) results suggested that
where all BSA was adsorbed, subsequent multiple pore volume microsphere
breakthrough curves reflected progressively reduced colloid deposition rates with
increasing adsorbed BSA content. Modelling colloid responses suggested adsorption
of 1 µg BSA generated the same response as blockage by between 7.1x108 and
2.3x109 deposited microspheres. By contrast, microsphere responses in DPEs where
BSA coverage of the deposition sites approached/ reached saturation revealed the
coated sand maintained a finite capacity to attenuate microspheres, even when
incapable of further BSA adsorption. Subsequent microsphere breakthrough curves
demonstrated the matrix's colloid attenuation capacity progressively increased
with continued microsphere deposition. Experimental findings suggested BSA
adsorption on the sand surface approaching/ reaching saturation generated
attractive deposition sites for colloids, which became progressively more
attractive with further colloid deposition (filter ripening). Results demonstrate
that adsorption of a single type of protein may either enhance or inhibit colloid
mobility in saturated porous media.

PMID: 22296282 [PubMed - as supplied by publisher]