albumin - publications

Predict more albumin - ligand interactions now!

1. Colloids Surf B Biointerfaces. 2012 Feb 16. [Epub ahead of print]

Adsorption of bovine serum albumin on nano and bulk oxide particles in deionized

Song L, Yang K, Jiang W, Du P, Xing B.

Department of Plant, Soil and Insect Sciences, University of Massachusetts,
Amherst, MA 01003, USA.

In this work, the influence of particle size and surface functional groups on the
adsorption behavior of bovine serum albumin (BSA) by three types of oxide
nanoparticles (NPs), TiO(2) (50±5nm), SiO(2) (30±5nm), and Al(2)O(3) (150±5nm for
α type and 60±5nm for γ type) was investigated in deionized water, in order to
explore their interaction mechanisms without competitive influence of other ions.
Bulkparticles (BPs) were also used for comparison with NPs. BSA adsorption maxima
on oxide particles were controlled by the surface area and hydrogen content,
while adsorption process was primarily induced by electrostatic interaction,
hydrophobic interaction and ligand exchange between BSA and oxide surfaces. With
the increase of hydrogen content, the BSA adsorption mechanism switched from
mainly hydrophobic interaction to hydrogen bonding and ligand exchange.
Calculations, based on surface area and BSA size, suggested that a multilayer of
BSA covered on α-Al(2)O(3), and single layer on the other oxide particle
surfaces. BPs led to greater conformational change of BSA molecules after the
adsorption on the surfaces of oxide particles though NPs adsorbed more BSA than

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22405471 [PubMed - as supplied by publisher]