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A novel electrochemiluminescence ethanol biosensor based on tris(2,2'-bipyridine) ruthenium (II) and alcohol dehydrogenase immobilized in graphene/bovine serum albumin composite film.


Biosens Bioelectron. 2012 Oct 11;


Authors: Gao W, Chen Y, Xi J, Lin S, Chen Y, Lin Y, Chen Z


Abstract

We developed a novel electrochemiluminescence (ECL) ethanol biosensor based on [Formula: see text] and alcohol dehydrogenase (ADH) immobilized by graphene/bovine serum albumin composite film. The graphene film was directly formed on a glassy carbon electrode surface via an in situ reduction of graphene oxide (GO) and [Formula: see text] was immobilized during its formation. The graphene film acted as both a decorating agent for immobilization of [Formula: see text] and a matrix to immobilize bovine serum albumin (BSA), meanwhile BSA not only acted as a reductant to reduce GO, but also provided a friendly environment for ADH immobilization. Furthermore, ADH was separated from [Formula: see text] by the electron-conductive graphene/BSA composite film to retain its enzymatic activity. The experimental results indicated that the biosensor had excellent electrochemical activity, ECL response to ethanol and stability. Such a design of [Formula: see text] -graphene/BSA film to modify electrode holds a great promise as a new biocompatible platform for the development of enzyme-based ECL biosensors.

PMID: 23122751 [PubMed - as supplied by publisher]