albumin - publications

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A new and simple resonance Rayleigh scattering method for human serum albumin using graphite oxide as probe.

Luminescence. 2012 Dec 17;

Authors: Wang S, Xu L, Wang L, Liang A, Jiang Z


Graphite oxide (GO) was prepared by the Hummer procedure, and can be dispersed to stable colloid solution by ultrasonic wave. The GO exhibited an absorption peak at 313 nm, and a resonance Rayleigh scattering (RRS) peak at 490 nm. In pH 4.6 HAc-NaAc buffer solution, human serum albumin (HSA) combined with GO probe to form large HSA-GO particles that caused the RRS peak increasing at 490 nm. The increased RRS intensity was linear to HSA concentration in the range 0.50-200 µg/mL. Thus, a new and simple RRS method was proposed for the determination of HSA in samples, with a recovery of 98.1-104%. Copyright © 2012 John Wiley & Sons, Ltd.

PMID: 23255403 [PubMed - as supplied by publisher]