albumin - publications

Predict more albumin - ligand interactions now!


1. J Pharm Biomed Anal. 2012 Jan 28. [Epub ahead of print]

A homogeneous fluorescent sensor for human serum albumin.

Wang RE, Tian L, Chang YH.

Mediomics, LLC, 5445 Highland Park Drive, St. Louis, MO 63110, USA.

Human serum albumin is the most abundant protein in the body and is an important
biomarker used for disease-related diagnosis. Although the traditional
enzyme-linked immunosorbent assay (ELISA) approach can precisely measure the
concentration of human serum albumin, the multi-step procedure and time-consuming
preparations of ELISA limit its diagnostic applications, preventing accurate
point-of-care testing, for example. Herein, we report the recent development of
an antibody-based albumin sensor that allows for a homogeneous measurement of
albumin concentrations in saliva, urine and serum, in which this type of sensor
is validated for the first time. The assay only requires simple mixing, and
relies on time-resolved (TR) fluorescence resonance energy transfer (FRET) to
produce robust, sensitive signals. The whole process, from sample preparation to
final read-out, is expected to take less than 1h and requires only a standard
plate-reader, thus making the sensor a convenient and cost-effective tool for
albumin analysis.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22326845 [PubMed - as supplied by publisher]